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pet28b cas9 his68  (Addgene inc)


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    Structured Review

    Addgene inc pet28b cas9 his68
    Pet28b Cas9 His68, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28b/pET-28b-Cas9-His+(Plasmid+%2347327)/bio_rxiv__64898__2026__03__18__712093-371-5-6
    Average 94 stars, based on 61 article reviews
    pet28b cas9 his68 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: A stochastic mechanism drives fast substrate translocation in the AAA+ machine ClpB
    Article Snippet: A double-mutant ClpB incorporated the unnatural amino acid N -ε-(prop-2-ynyloxycarbonyl)- L -lysine (ProK, Iris Biotech) at residue 771, and a cysteine at residue 176. .. ProK was coded with the Amber stop codon, allowing for co-transfection of the double mutant 176 C + 771TAG ClpB in pET28b together with pEvol PylRS/tRNApyl (Addgene, Plasmid #127411, back mutated to A346N/A348C) into E. Coli BL21 (DE3) pLysS cells (Invitrogen) using heat shock. .. Cells were recovered and incubated in 10 mL Luria Broth (LB, Thermo Fisher) media containing kanamycin (Sigma, 50 μg/mL) and chloramphenicol (Sigma, 25 μg/mL) for 16 h at 37 °C, 250 rpm.

    Article Title: Millisecond bidirectional protein translocation by a HSP100 machine
    Article Snippet: A double-mutant ClpB incorporated the unnatural amino acid N -ε-(prop-2- ynyloxycarbonyl)- L -lysine (ProK, Iris Biotech) at residue 771, and a cysteine at residue 176. .. ProK was coded with the Amber stop codon, allowing for co-transfection of the double mutant 176C + 771TAG ClpB in pET28b together with pEvol PylRS/tRNApyl (Addgene, Plasmid #127411, back mutated to A346N/A348C) into E. Coli BL21 (DE3) pLysS cells (Invitrogen) using heat shock. .. Cells were recovered and incubated in 10 mL Luria Broth (LB, Thermo Fisher) media containing kanamycin (Sigma, 50 μg/mL) and chloramphenicol (Sigma, 25 μg/mL) for 16 h at 37 °C, 250 rpm.

    Plasmid Preparation:

    Article Title: A stochastic mechanism drives fast substrate translocation in the AAA+ machine ClpB
    Article Snippet: A double-mutant ClpB incorporated the unnatural amino acid N -ε-(prop-2-ynyloxycarbonyl)- L -lysine (ProK, Iris Biotech) at residue 771, and a cysteine at residue 176. .. ProK was coded with the Amber stop codon, allowing for co-transfection of the double mutant 176 C + 771TAG ClpB in pET28b together with pEvol PylRS/tRNApyl (Addgene, Plasmid #127411, back mutated to A346N/A348C) into E. Coli BL21 (DE3) pLysS cells (Invitrogen) using heat shock. .. Cells were recovered and incubated in 10 mL Luria Broth (LB, Thermo Fisher) media containing kanamycin (Sigma, 50 μg/mL) and chloramphenicol (Sigma, 25 μg/mL) for 16 h at 37 °C, 250 rpm.

    Article Title: TBK1 Induces the Formation of Optineurin Filaments That Condensate with Polyubiquitin and LC3 for Cargo Sequestration
    Article Snippet: .. The following plasmids were obtained from Addgene: pEGFP‐N1‐OPTN‐WT (Addgene plasmid number: 27052), pEGFP‐N1‐OPTN‐Q398X(Addgene plasmid number: 68849), pEGFP‐N1‐OPTN‐E478G (Addgene number: 68848), pET28b His‐mouse UBE1 (Addgene plasmid number: 32534), pET28a‐LIC UBE2V1 (Addgene Plasmid number: 25619), pOPINF‐OTULIN (Addgene plasmid number: 61464). .. The pET9‐His‐M1‐linked octa‐ubiquitin, to which the SGCG residues were added in frame after the C‐terminal ubiquitin, was codon‐optimised and gene‐synthesised (Genscript).

    Article Title: Multivalent OspA polypeptides and methods and uses relating thereto
    Article Snippet: .. In a preferred embodiment, the vector is pET28b(+) (http://www.addgene.org/vector-database/2566/). ..

    Article Title: Millisecond bidirectional protein translocation by a HSP100 machine
    Article Snippet: A double-mutant ClpB incorporated the unnatural amino acid N -ε-(prop-2- ynyloxycarbonyl)- L -lysine (ProK, Iris Biotech) at residue 771, and a cysteine at residue 176. .. ProK was coded with the Amber stop codon, allowing for co-transfection of the double mutant 176C + 771TAG ClpB in pET28b together with pEvol PylRS/tRNApyl (Addgene, Plasmid #127411, back mutated to A346N/A348C) into E. Coli BL21 (DE3) pLysS cells (Invitrogen) using heat shock. .. Cells were recovered and incubated in 10 mL Luria Broth (LB, Thermo Fisher) media containing kanamycin (Sigma, 50 μg/mL) and chloramphenicol (Sigma, 25 μg/mL) for 16 h at 37 °C, 250 rpm.

    Article Title: Mutant fragments of OspA and methods and uses relating thereto
    Article Snippet: For example, peptide synthesis can be performed using various solid-phase techniques (Roberge et al., Science 269:202-204 (1995)) and automated synthesis may be achieved, for example, using the ASI 431 A Peptide Synthesizer (Perkin Elmer, Palo Alto, Calif.). .. In a preferred embodiment, the vector is pET28b(+) (http://www.addgene.org/vector-database/2566/). ..

    Expressing:

    Article Title: Supporting Information for Animal FAS-like polyketide synthases produce diverse polypropionates
    Article Snippet: .. Expression and purification of MatB, ACS, UkaQ and Sfp. matB and UkaQ were synthesized by Genewiz and cloned into pET28b at NocI and XhoI sites. pET-acs and pET-sfps were purchased from Addgene. ..

    Purification:

    Article Title: Supporting Information for Animal FAS-like polyketide synthases produce diverse polypropionates
    Article Snippet: .. Expression and purification of MatB, ACS, UkaQ and Sfp. matB and UkaQ were synthesized by Genewiz and cloned into pET28b at NocI and XhoI sites. pET-acs and pET-sfps were purchased from Addgene. ..

    Synthesized:

    Article Title: Supporting Information for Animal FAS-like polyketide synthases produce diverse polypropionates
    Article Snippet: .. Expression and purification of MatB, ACS, UkaQ and Sfp. matB and UkaQ were synthesized by Genewiz and cloned into pET28b at NocI and XhoI sites. pET-acs and pET-sfps were purchased from Addgene. ..

    Clone Assay:

    Article Title: Supporting Information for Animal FAS-like polyketide synthases produce diverse polypropionates
    Article Snippet: .. Expression and purification of MatB, ACS, UkaQ and Sfp. matB and UkaQ were synthesized by Genewiz and cloned into pET28b at NocI and XhoI sites. pET-acs and pET-sfps were purchased from Addgene. ..



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